No correlation was observed in IgM and IgG levels of ELISA and FIA assays. Moreover, the IgM and IgG performance of FIA had higher specificity, PPV, and agreement than the IgM IC performance, suggesting that the FIA was more specific but less sensitive for antibody detection. Results showed that the viral nonstructural protein (NS1) performance of FIA was slightly higher than IC with the sensitivity, specificity, PPV, NPV, agreement, kappa, and its standard error at 79.11, 92.28, 86.81, 87.31, 352 (87.13%), 0.725 ± 0.035, respectively whereas those of the IC were at 76.58, 92.28, 86.43, 85.98, 348 (86.14%), 0.703 ± 0.037, respectively. The performance of both tests was verified by a definitive diagnosis retrieved from combinatorial reverse transcription-quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) for IgM and IgG confirmation tests. This report recruited samples with acute febrile illnesses sent for dengue screening and tested IC and FIA in parallel. Fluorescent immunoassay (FIA) utilized a highly sensitive detection system and claimed 70–100% sensitivity and 83.5–91.7% specificity for dengue infection in a preliminary report. Currently, dengue immunochromatography (IC) is commonly used to primarily differentiate acute febrile illnesses. Therefore, a rapid and accurate diagnosis is critical for an appropriate management as it could reduce the burden of severe clinical manifestation. Dengue virus (DENV 1–4) infection has been a global health threat where no specific treatment is currently available.
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